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验证数据展示

  • WB analysis using 68043-1-Ig (same clone as 68043-1-PBS)

    WB analysis using 68043-1-Ig (same clone as 68043-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 68043-1-Ig (same clone as 68043-1-PBS)

    IHC staining of mouse brain using 68043-1-Ig (same clone as 68043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SYT1 antibody included
  • IHC staining of mouse brain using 68043-1-Ig (same clone as 68043-1-PBS)

    IHC staining of mouse brain using 68043-1-Ig (same clone as 68043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SYT1 antibody included

Synaptotagmin-1 Monoclonal antibody, PBS Only

Synaptotagmin-1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68043-1-PBS

产品说明书

CloneNo. 1H1H2

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Pig, Rabbit, Chicken

应用

WB, IHC, Indirect ELISA

别名

DKFZp781D2042, P65, SVP65, Synaptotagmin 1, synaptotagmin I, Synaptotagmin-1, SYT, SYT1, SytI

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SYT1 Ready-To-Use IHC Kit. SYT1 primary antibody included.

产品信息

68043-1-PBS targets Synaptotagmin-1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit, Chicken samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig, Rabbit, Chicken
免疫原 Synaptotagmin-1 fusion protein Ag6071 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 synaptotagmin I
别名 DKFZp781D2042, P65, SVP65, Synaptotagmin 1, synaptotagmin I, Synaptotagmin-1, SYT, SYT1, SytI
计算分子量 48 kDa
观测分子量60-65 kDa
GenBank蛋白编号BC058917
基因名称 Synaptotagmin-1
Gene ID (NCBI) 6857
RRIDAB_2918785
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP21579
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT1 (synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue (PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse (PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth (PMID: 15492212; 11078930; 10336114).