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Synaptotagmin-1 Monoclonal antibody

Synaptotagmin-1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit, Chicken

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H1H2

Cat no : 68043-1-Ig

Print datasheet

Synonyms

DKFZp781D2042, P65, SVP65, Synaptotagmin 1, synaptotagmin I, Synaptotagmin-1, SYT, SYT1, SytI

验证数据展示

  • WB analysis using 68043-1-Ig

    WB analysis using 68043-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 68043-1-Ig

    IHC staining of mouse brain using 68043-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SYT1 antibody included
  • IHC staining of mouse brain using 68043-1-Ig

    IHC staining of mouse brain using 68043-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SYT1 antibody included

经过测试的应用

Positive WB detected inpig brain tissue, rat cerebellum tissue, mouse cerebellum tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue, rabbit cerebellum tissue
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SYT1 Ready-To-Use IHC Kit. SYT1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68043-1-Ig targets Synaptotagmin-1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit, Chicken samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig, Rabbit, Chicken
Immunogen Synaptotagmin-1 fusion protein Ag6071 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name synaptotagmin I
Synonyms DKFZp781D2042, P65, SVP65, Synaptotagmin 1, synaptotagmin I, Synaptotagmin-1, SYT, SYT1, SytI
Calculated Molecular Weight 48 kDa
Observed Molecular Weight60-65 kDa
GenBank Accession NumberBC058917
Gene Symbol Synaptotagmin-1
Gene ID (NCBI) 6857
RRIDAB_2918785
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP21579
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT1 (synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue (PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse (PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth (PMID: 15492212; 11078930; 10336114).

实验方案

Product Specific Protocols
WB protocol for Synaptotagmin-1 antibody 68043-1-IgDownload protocol
IHC protocol for Synaptotagmin-1 antibody 68043-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols