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验证数据展示

  • FC experiment of SH-SY5Y using CL488-68043

    FC experiment of SH-SY5Y using CL488-68043

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated Synaptotagmin-1 Monoclonal antibody (CL488-68043, Clone:1H1H2)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated Synaptotagmin-1 Monoclonal antibody

Synaptotagmin-1 Monoclonal Antibody for FC (Intra)
Cat No. CL488-68043

产品说明书

CloneNo. 1H1H2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit, chicken

应用

FC (Intra)

别名

Synaptotagmin 1, SYT1, SYT, synaptotagmin I, SVP65

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy SYT1 Ready-To-Use IHC Kit. SYT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68043-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68043 targets Synaptotagmin-1 in FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig, rabbit, chicken
免疫原 Synaptotagmin-1 fusion protein Ag6071 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 synaptotagmin I
别名 Synaptotagmin 1, SYT1, SYT, synaptotagmin I, SVP65
计算分子量 48 kDa
观测分子量60-65 kDa
GenBank蛋白编号BC058917
基因名称 Synaptotagmin-1
Gene ID (NCBI) 6857
RRIDAB_2923868
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP21579
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT1 (synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue (PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse (PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth (PMID: 15492212; 11078930; 10336114).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 Synaptotagmin-1 antibody CL488-68043Download protocol
Standard Protocols
Click here to view our Standard Protocols