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Synaptotagmin-9 Recombinant antibody

Synaptotagmin-9 Uni-rAbTM Recombinant Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

241407A11

Cat no : 84123-3-RR

Print datasheet

Synonyms

SytIX, SYT9, Synaptotagmin IX, 241407A11

验证数据展示

  • WB analysis of mouse eye using 84123-3-RR

    WB analysis of mouse eye using 84123-3-RR

    mouse eye tissue was subjected to SDS PAGE followed by western blot with 84123-3-RR (Synaptotagmin-9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse retina using 84123-3-RR

    IF Staining of mouse retina using 84123-3-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse retina tissue using Synaptotagmin-9 antibody (84123-3-RR, Clone: 241407A11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84123-3-RR

    Affinity and Kinetic Characterization of 84123-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84123-3-RR against Human Synaptotagmin-9 were performed. The affinity constant is 22.5 nM.

经过测试的应用

Positive WB detected inmouse eye tissue
Positive IF-P detected inmouse retina tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84123-3-RR targets Synaptotagmin-9 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Synaptotagmin-9 fusion protein Ag3520 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name synaptotagmin IX
Synonyms SytIX, SYT9, Synaptotagmin IX, 241407A11
Calculated Molecular Weight 491 aa, 52 kDa
Observed Molecular Weight52-56 kDa
GenBank Accession NumberBC029605
Gene Symbol Synaptotagmin-9
Gene ID (NCBI) 143425
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ86SS6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-9 (SYT9) is a tandem C2 domain Ca 2+ sensor for exocytosis in neuroendocrine cells (PMID: 36732068).

实验方案

Product Specific Protocols
WB protocol for Synaptotagmin-9 antibody 84123-3-RRDownload protocol
IF protocol for Synaptotagmin-9 antibody 84123-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols