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验证数据展示

  • WB analysis of mouse eye using 84123-3-RR

    WB analysis of mouse eye using 84123-3-RR

    mouse eye tissue was subjected to SDS PAGE followed by western blot with 84123-3-RR (Synaptotagmin-9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse retina using 84123-3-RR

    IF Staining of mouse retina using 84123-3-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse retina tissue using Synaptotagmin-9 antibody (84123-3-RR, Clone: 241407A11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84123-3-RR

    Affinity and Kinetic Characterization of 84123-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84123-3-RR against Human Synaptotagmin-9 were performed. The affinity constant is 22.5 nM.

Synaptotagmin-9 Recombinant antibody

Synaptotagmin-9 Uni-rAbTM Recombinant Antibody for WB, IF-P, ELISA
Cat No. 84123-3-RR

产品说明书

CloneNo. 241407A11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-P, ELISA

别名

SytIX, SYT9, Synaptotagmin IX, 241407A11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse eye tissue
Positive IF-P detected inmouse retina tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84123-3-RR targets Synaptotagmin-9 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 Synaptotagmin-9 fusion protein Ag3520 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 synaptotagmin IX
别名 SytIX, SYT9, Synaptotagmin IX, 241407A11
计算分子量 491 aa, 52 kDa
观测分子量52-56 kDa
GenBank蛋白编号BC029605
基因名称 Synaptotagmin-9
Gene ID (NCBI) 143425
RRIDAB_3671684
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ86SS6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-9 (SYT9) is a tandem C2 domain Ca 2+ sensor for exocytosis in neuroendocrine cells (PMID: 36732068).

实验方案

Product Specific Protocols
WB protocol for Synaptotagmin-9 antibody 84123-3-RRDownload protocol
IF protocol for Synaptotagmin-9 antibody 84123-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols