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验证数据展示

  • WB analysis of mouse eye using 84123-3-RR (same clone as 84123-3-PBS)

    WB analysis of mouse eye using 84123-3-RR (same clone as 84123-3-PBS)

    mouse eye tissue was subjected to SDS PAGE followed by western blot with 84123-3-RR (Synaptotagmin-9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84123-3-PBS in a different storage buffer formulation.

  • IF Staining of mouse retina using 84123-3-RR (same clone as 84123-3-PBS)

    IF Staining of mouse retina using 84123-3-RR (same clone as 84123-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse retina tissue using Synaptotagmin-9 antibody (84123-3-RR, Clone: 241407A11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84123-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84123-3-RR

    Affinity and Kinetic Characterization of 84123-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84123-3-RR against Human Synaptotagmin-9 were performed. The affinity constant is 22.5 nM.

Synaptotagmin-9 Recombinant monoclonal antibody, PBS Only

Synaptotagmin-9 Uni-rAb® Recombinant Antibody for WB, IF-P, Indirect ELISA
Cat No. 84123-3-PBS

产品说明书

CloneNo. 241407A11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-P, Indirect ELISA

别名

SytIX, SYT9, Synaptotagmin IX, 241407A11

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84123-3-PBS targets Synaptotagmin-9 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag3520

Product name: Recombinant human SYT9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 45-319 aa of BC029605

Sequence: LCWVPWRERGLPSGSKDNNQEPLNYMDTETNEQENSEDFLDPPTPCPDSSMKISHTSPDIPLSTQTGIQENCAHGVRVQRQVTEPTSSARHNSIRRQLNLSNPDFNIQQLQKQEQLTGIGRIKPELYKQRSLDNDDGRRSNSKACGKLNFILKYDCDLEQLIVKIHKAVNLPAKDFSGTSDPYVKIYLLPDRKTKHQTKVHRKTLNPVFDEVFLFPVPYNDLEARKLHFSVYDFDRFSRHDLIGQVVVDHFLDLADFPRECILWKDIEYVTNILR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 synaptotagmin IX
别名 SytIX, SYT9, Synaptotagmin IX, 241407A11
计算分子量 491 aa, 52 kDa
观测分子量52-56 kDa
GenBank蛋白编号BC029605
基因名称 Synaptotagmin-9
Gene ID (NCBI) 143425
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ86SS6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-9 (SYT9) is a tandem C2 domain Ca 2+ sensor for exocytosis in neuroendocrine cells (PMID: 36732068).

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