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验证数据展示

  • IF Staining of mouse brain using CL488-66437

    IF Staining of mouse brain using CL488-66437

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Syntaxin 1A / Syntaxin 1B antibody (CL488-66437, Clone: 2D3B5 ) at dilution of 1:200.

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  • KD/KO验证

CoraLite® Plus 488-conjugated Syntaxin 1A / Syntaxin 1B Monoclonal antibody

Syntaxin 1A / Syntaxin 1B Monoclonal Antibody for IF-P
Cat No. CL488-66437

产品说明书

CloneNo. 2D3B5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF-P

别名

STX1B, STX1B1, STX1B2, Syntaxin 1A / Syntaxin 1B, syntaxin 1B, Syntaxin 1B1, Syntaxin 1B2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy Syntaxin 1A/1B Ready-To-Use IHC Kit. Syntaxin 1A/1B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66437-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66437 targets Syntaxin 1A / Syntaxin 1B in IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 Syntaxin 1A / Syntaxin 1B fusion protein Ag10257 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 syntaxin 1B
别名 STX1B, STX1B1, STX1B2, Syntaxin 1A / Syntaxin 1B, syntaxin 1B, Syntaxin 1B1, Syntaxin 1B2
计算分子量 288 aa, 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC062298
基因名称 STX1B
Gene ID (NCBI) 112755
RRIDAB_2919324
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP61266
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and the endocytic pathway (PMID: 11737951). Syntaxin 1A and 1B, two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Syntaxin 1A / Syntaxin 1B antibody CL488-66437Download protocol
Standard Protocols
Click here to view our Standard Protocols