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验证数据展示

  • IF Staining of mouse brain using CL488-66437

    IF Staining of mouse brain using CL488-66437

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Syntaxin 1A / Syntaxin 1B antibody (CL488-66437, Clone: 2D3B5 ) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Syntaxin 1A / Syntaxin 1B Monoclonal antibody

Syntaxin 1A / Syntaxin 1B Monoclonal Antibody for IF-P
Cat No. CL488-66437

Print datasheet

CloneNo. 2D3B5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF-P

Synonyms

STX1B, STX1B1, STX1B2, Syntaxin 1A / Syntaxin 1B, syntaxin 1B, Syntaxin 1B1, Syntaxin 1B2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy Syntaxin 1A/1B Ready-To-Use IHC Kit. Syntaxin 1A/1B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66437-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66437 targets Syntaxin 1A / Syntaxin 1B in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen Syntaxin 1A / Syntaxin 1B fusion protein Ag10257 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name syntaxin 1B
Synonyms STX1B, STX1B1, STX1B2, Syntaxin 1A / Syntaxin 1B, syntaxin 1B, Syntaxin 1B1, Syntaxin 1B2
Calculated Molecular Weight 288 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC062298
Gene Symbol STX1B
Gene ID (NCBI) 112755
RRIDAB_2919324
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP61266
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and the endocytic pathway (PMID: 11737951). Syntaxin 1A and 1B, two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Syntaxin 1A / Syntaxin 1B antibody CL488-66437Download protocol
Standard Protocols
Click here to view our Standard Protocols