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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Syntaxin 6 Polyclonal antibody

Syntaxin 6 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10841

Print datasheet

Synonyms

Syntaxin-6, STX6

验证数据展示

  • IF Staining of PC-12 using CL594-10841

    IF Staining of PC-12 using CL594-10841

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using CoraLite®594 Syntaxin 6 antibody (CL594-10841) at dilution of 1:100.

  • FC experiment of PC-12 using CL594-10841

    FC experiment of PC-12 using CL594-10841

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Syntaxin 6 (CL594-10841) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inPC-12 cells
Positive FC (Intra) detected inPC-12 cells
For other applications, we recommend the unconjugated version of this antibody, 10841-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-10841 targets Syntaxin 6 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Syntaxin 6 fusion protein Ag1284 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name syntaxin 6
Synonyms Syntaxin-6, STX6
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC009944
Gene Symbol Syntaxin 6
Gene ID (NCBI) 10228
RRIDAB_2919749
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43752
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.

实验方案

Product Specific Protocols
IF protocol for CL594 Syntaxin 6 antibody CL594-10841Download protocol
FC protocol for CL594 Syntaxin 6 antibody CL594-10841Download protocol
Standard Protocols
Click here to view our Standard Protocols