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验证数据展示

  • FC experiment of HepG2 using CL488-68096

    FC experiment of HepG2 using CL488-68096

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Syntenin-1 (CL488-68096, Clone:2B1A9) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated Syntenin-1 Monoclonal antibody

Syntenin-1 Monoclonal Antibody for FC (Intra)
Cat No. CL488-68096

产品说明书

CloneNo. 2B1A9

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC (Intra)

别名

SDCBP, Pro-TGF-alpha cytoplasmic domain-interacting protein 18, Melanoma differentiation-associated protein 9, MDA-9, MDA9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SDCBP Ready-To-Use IHC Kit. SDCBP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68096-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68096 targets Syntenin-1 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 Syntenin-1 fusion protein Ag18078 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 syndecan binding protein (syntenin)
别名 SDCBP, Pro-TGF-alpha cytoplasmic domain-interacting protein 18, Melanoma differentiation-associated protein 9, MDA-9, MDA9
计算分子量 298 aa, 32 kDa
观测分子量 30 kDa
GenBank蛋白编号BC113674
基因名称 Syntenin-1
Gene ID (NCBI) 6386
RRIDAB_3084398
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO00560
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syntenin-1, also known as SDCBP (syndecan binding protein) and MDA-9 (melanoma differentiation-associated protein 9), was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. Syntenin-1 is a PDZ-domain-containing molecule that has many interaction partners, and regulates transmembrane-receptor trafficking, cell adhesion, tumor-cell metastasis and neuronal-synapse function. Syntenin-1 is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 Syntenin-1 antibody CL488-68096Download protocol
Standard Protocols
Click here to view our Standard Protocols