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TACSTD2/TROP2 Polyclonal antibody

TACSTD2/TROP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 27360-1-AP

Print datasheet

Synonyms

EGP 1, GA733, GA733 1, M1S1, TACSTD2, TROP2

验证数据展示

  • WB analysis of HaCaT using 27360-1-AP

    WB analysis of HaCaT using 27360-1-AP

    HaCaT cells were subjected to SDS PAGE followed by western blot with 27360-1-AP (TACSTD2 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 27360-1-AP

    IHC staining of human breast cancer using 27360-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27360-1-AP (TACSTD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TACSTD2 antibody included
  • IHC staining of human breast cancer using 27360-1-AP

    IHC staining of human breast cancer using 27360-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27360-1-AP (TACSTD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TACSTD2 antibody included
  • IF Staining of HaCaT using 27360-1-AP

    IF Staining of HaCaT using 27360-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using TACSTD2/TROP2 antibody (27360-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHaCaT cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHaCaT cells
Planning an IHC experiment? We recommend our IHCeasy TACSTD2 Ready-To-Use IHC Kit. TACSTD2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27360-1-AP targets TACSTD2/TROP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen TACSTD2/TROP2 fusion protein Ag25769 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tumor-associated calcium signal transducer 2
Synonyms EGP 1, GA733, GA733 1, M1S1, TACSTD2, TROP2
Calculated Molecular Weight 36 kDa
Observed Molecular Weight45-55 kDa
GenBank Accession NumberBC009409
Gene Symbol TROP2
Gene ID (NCBI) 4070
RRIDAB_2918122
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09758
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Trophoblast cell surface antigen 2 (TROP2), also named as TACSTD2, is a transmembrane glycoprotein. TROP2 plays a role in transducing intracellular calcium signals. It is expressed in trophoblast cells, cornea and multi-stratified epithelia. TROP2 is overexpressed in most carcinomas and is involved in cancer proliferation, migration, invasion, and metastasis. Congenital mutations of TROP2 cause a rare autosomal recessive disease which may lead to blindness-the gelatinous drop-like corneal disease (PMID: 35688908).

实验方案

Product Specific Protocols
WB protocol for TACSTD2/TROP2 antibody 27360-1-APDownload protocol
IHC protocol for TACSTD2/TROP2 antibody 27360-1-APDownload protocol
IF protocol for TACSTD2/TROP2 antibody 27360-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

J Adv Res

Targeting Trop2 by Bruceine D suppresses breast cancer metastasis by blocking Trop2/β-catenin positive feedback loop

Authors - Wenjuan Tang
View Article
humanWB

J Extracell Vesicles

PD-L1 on large extracellular vesicles is a predictive biomarker for therapy response in tissue PD-L1-low and -negative patients with non-small cell lung cancer

Authors - Nadja Schöne
View Article