验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HaCaT cells |
Planning an IHC experiment? We recommend our IHCeasy TACSTD2 Ready-To-Use IHC Kit. TACSTD2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68141-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68141 targets TACSTD2/TROP2 in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 | TACSTD2/TROP2 fusion protein Ag25775 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | tumor-associated calcium signal transducer 2 |
| 别名 | TACSTD2, GA733-1, GA733 1, GA733, EGP 1 |
| 计算分子量 | 36 kDa |
| 观测分子量 | 40-50 kDa |
| GenBank蛋白编号 | BC009409 |
| 基因名称 | TROP2 |
| Gene ID (NCBI) | 4070 |
| RRID | AB_3672974 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P09758 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Trophoblast cell surface antigen 2 (TROP2), also named as TACSTD2, is a transmembrane glycoprotein. TROP2 plays a role in transducing intracellular calcium signals. It is expressed in trophoblast cells, cornea and multi-stratified epithelia. TROP2 is overexpressed in most carcinomas and is involved in cancer proliferation, migration, invasion, and metastasis. Congenital mutations of TROP2 cause a rare autosomal recessive disease which may lead to blindness-the gelatinous drop-like corneal disease (PMID: 35688908).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 TACSTD2/TROP2 antibody CL488-68141 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |