验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, K-562 cells, MCF-7 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
产品信息
17367-1-AP targets TADA2B in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | TADA2B fusion protein Ag10783 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transcriptional adaptor 2 (ADA2 homolog, yeast)-beta |
| 别名 | ADA2-like protein beta, ADA2-beta, ADA2B, ADA2(beta), ADA2 like protein beta |
| 计算分子量 | 420 aa, 48 kDa |
| 观测分子量 | 55-62 kDa |
| GenBank蛋白编号 | BC013164 |
| 基因名称 | TADA2B |
| Gene ID (NCBI) | 93624 |
| RRID | AB_2201049 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86TJ2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TADA2B antibody 17367-1-AP | Download protocol |
| IHC protocol for TADA2B antibody 17367-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv The KAT module of the SAGA complex maintains the oncogenic gene expression program in MYCN-amplified neuroblastoma |