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验证数据展示

  • FC experiment of HepG2 using CL488-67569

    FC experiment of HepG2 using CL488-67569

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TAF6L (CL488-67569, Clone:1G5C4) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated TAF6L Monoclonal antibody

TAF6L Monoclonal Antibody for FC (Intra)
Cat No. CL488-67569

产品说明书

CloneNo. 1G5C4

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat

应用

FC (Intra)

别名

TAF6L, TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L, 1G5C4, PAF65 alpha, PAF65A, PAF65-alpha, PCAF-associated factor 65-alpha

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67569-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67569 targets TAF6L in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, mouse, rat
免疫原 TAF6L fusion protein Ag8202 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 TAF6-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kDa
别名 TAF6L, TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L, 1G5C4, PAF65 alpha, PAF65A, PAF65-alpha, PCAF-associated factor 65-alpha
计算分子量 622 aa, 68 kDa
观测分子量 68 kDa
GenBank蛋白编号BC008785
基因名称 TAF6L
Gene ID (NCBI) 10629
RRIDAB_2923777
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y6J9
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 TAF6L antibody CL488-67569Download protocol
Standard Protocols
Click here to view our Standard Protocols