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TAK1 Monoclonal antibody, PBS Only

TAK1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A5B2

Cat no : 67707-1-PBS

Print datasheet

Synonyms

MAP3K7, TAK1, TGF beta activated kinase 1, TGF1a

验证数据展示

  • WB analysis using 67707-1-Ig (same clone as 67707-1-PBS)

    WB analysis using 67707-1-Ig (same clone as 67707-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67707-1-Ig (TAK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 67707-1-Ig (same clone as 67707-1-PBS)

    IHC staining of human prostate cancer using 67707-1-Ig (same clone as 67707-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAP3K7/TAK1 antibody included
  • IF Staining of HepG2 using 67707-1-Ig (same clone as 67707-1-PBS)

    IF Staining of HepG2 using 67707-1-Ig (same clone as 67707-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TAK1 antibody (67707-1-Ig, Clone: 1A5B2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

  • WB analysis using 67707-1-Ig (same clone as 67707-1-PBS)

    WB analysis using 67707-1-Ig (same clone as 67707-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67707-1-Ig (TAK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67707-1-Ig (same clone as 67707-1-PBS)

    IF Staining of HepG2 using 67707-1-Ig (same clone as 67707-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TAK1 antibody (67707-1-Ig, Clone: 1A5B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MAP3K7/TAK1 Ready-To-Use IHC Kit. MAP3K7/TAK1 primary antibody included.

产品信息

67707-1-PBS targets TAK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen TAK1 fusion protein Ag21286 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase kinase 7
Synonyms MAP3K7, TAK1, TGF beta activated kinase 1, TGF1a
Calculated Molecular Weight 579 aa, 64 kDa
Observed Molecular Weight67-70 kDa
GenBank Accession NumberBC017715
Gene Symbol TAK1
Gene ID (NCBI) 6885
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43318
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named as TAK1 and belongs to the MAP kinase kinase kinase subfamily. It plays an important role in the cascades of cellular responses evoked by changes in the environment. It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825). It has 4 isoforms (53-55 kDa, 64-70 kDa and 75-80 kDa) produced by alternative splicing.