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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67707-1-Ig (same clone as 67707-1-PBS)

    WB analysis using 67707-1-Ig (same clone as 67707-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67707-1-Ig (TAK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 67707-1-Ig (same clone as 67707-1-PBS)

    IHC staining of human prostate cancer using 67707-1-Ig (same clone as 67707-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67707-1-Ig (TAK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAP3K7/TAK1 antibody included
  • IF Staining of HepG2 using 67707-1-Ig (same clone as 67707-1-PBS)

    IF Staining of HepG2 using 67707-1-Ig (same clone as 67707-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TAK1 antibody (67707-1-Ig, Clone: 1A5B2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 67707-1-Ig (same clone as 67707-1-PBS)

    FC experiment of K-562 using 67707-1-Ig (same clone as 67707-1-PBS)

    1x10^6 K-562 cells were intracellularly stained with 0.2 μg TAK1 Monoclonal antibody (67707-1-Ig, Clone:1A5B2 (red) or Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3 (blue), and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

  • WB analysis using 67707-1-Ig (same clone as 67707-1-PBS)

    WB analysis using 67707-1-Ig (same clone as 67707-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67707-1-Ig (TAK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67707-1-Ig (same clone as 67707-1-PBS)

    IF Staining of HepG2 using 67707-1-Ig (same clone as 67707-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TAK1 antibody (67707-1-Ig, Clone: 1A5B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67707-1-PBS in a different storage buffer formulation.

TAK1 Monoclonal antibody, PBS Only

TAK1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 67707-1-PBS

产品说明书

CloneNo. 1A5B2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

MAP3K7, Mitogen-activated protein kinase kinase kinase 7, mitogen activated protein kinase kinase kinase 7, MAP3K 7, EC:2.7.11.25

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MAP3K7/TAK1 Ready-To-Use IHC Kit. MAP3K7/TAK1 primary antibody included.

产品信息

67707-1-PBS targets TAK1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TAK1 fusion protein Ag21286 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mitogen-activated protein kinase kinase kinase 7
别名 MAP3K7, Mitogen-activated protein kinase kinase kinase 7, mitogen activated protein kinase kinase kinase 7, MAP3K 7, EC:2.7.11.25
计算分子量 579 aa, 64 kDa
观测分子量67-70 kDa
GenBank蛋白编号BC017715
基因名称 TAK1
Gene ID (NCBI) 6885
RRIDAB_2882898
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO43318
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named as TAK1 and belongs to the MAP kinase kinase kinase subfamily. It plays an important role in the cascades of cellular responses evoked by changes in the environment. It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825). It has 4 isoforms (53-55 kDa, 64-70 kDa and 75-80 kDa) produced by alternative splicing.