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验证数据展示

  • IF Staining of HepG2 using CL594-67707

    IF Staining of HepG2 using CL594-67707

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 TAK1 antibody (CL594-67707, Clone: 1A5B2 ) at dilution of 1:200.

CoraLite®594-conjugated TAK1 Monoclonal antibody

TAK1 Monoclonal Antibody for IF/ICC
Cat No. CL594-67707

产品说明书

CloneNo. 1A5B2

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

IF/ICC

别名

MAP3K7, TAK1, TGF beta activated kinase 1, TGF1a

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MAP3K7/TAK1 Ready-To-Use IHC Kit. MAP3K7/TAK1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67707-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67707 targets TAK1 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Mouse, Rat
免疫原 TAK1 fusion protein Ag21286 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mitogen-activated protein kinase kinase kinase 7
别名 MAP3K7, TAK1, TGF beta activated kinase 1, TGF1a
计算分子量 579 aa, 64 kDa
观测分子量67-70 kDa
GenBank蛋白编号BC017715
基因名称 TAK1
Gene ID (NCBI) 6885
RRIDAB_3673522
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO43318
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named as TAK1 and belongs to the MAP kinase kinase kinase subfamily. It plays an important role in the cascades of cellular responses evoked by changes in the environment. It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825). It has 4 isoforms (53-55 kDa, 64-70 kDa and 75-80 kDa) produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL594 TAK1 antibody CL594-67707Download protocol
Standard Protocols
Click here to view our Standard Protocols