验证数据展示
经过测试的应用
Positive WB detected in | A2780 cells, SW 1990 cells, HeLa cels, MDA-MB-231 cells, HaCaT cells, THP-1 cells, Daudi cells, Ramos cells, Raji cells |
Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
68412-1-Ig targets TAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | Human |
文献引用反应性 | human |
免疫原 | TAP1 fusion protein Ag21303 种属同源性预测 |
宿主/亚型 | Mouse / IgG2b |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) |
别名 | ABC17, ABCB2, Antigen peptide transporter 1, APT1, D6S114E, FLJ26666, FLJ41500, Peptide supply factor 1, Peptide transporter PSF1, Peptide transporter TAP1, PSF 1, PSF1, RING4, TAP1, TAP1*0102N, TAP1N, Y3 |
计算分子量 | 81 kDa |
观测分子量 | 70 kDa |
GenBank蛋白编号 | BC014081 |
基因名称 | TAP1 |
Gene ID (NCBI) | 6890 |
RRID | AB_3085128 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q03518 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TAP1, also known as ABCB2, PSF1 or RING4, is a member of the ATP-binding cassette (ABC) family of transmembrane transporters and is an essential component of the major histocompatability complex (MHC) class I antigen-presenting pathway. TAP is involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It also acts as a molecular scaffold for the final stage of MHC class I folding. Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1). Western blot analysis using this antibody detected a major band around 70 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for TAP1 antibody 68412-1-Ig | Download protocol |
IHC protocol for TAP1 antibody 68412-1-Ig | Download protocol |
IF protocol for TAP1 antibody 68412-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |