TAP1 Monoclonal antibody

TAP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68412-1-Ig

产品说明书

CloneNo. 1B8A7

宿主/亚型

Mouse / IgG2b

种属反应性

Human and More (1)

应用

WB, IHC, IF/ICC, ELISA

ABC17, ABCB2, Antigen peptide transporter 1, APT1, D6S114E, FLJ26666, FLJ41500, Peptide supply factor 1, Peptide transporter PSF1, Peptide transporter TAP1, PSF 1, PSF1, RING4, TAP1, TAP1*0102N, TAP1N, Y3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA2780 cells, SW 1990 cells, HeLa cels, MDA-MB-231 cells, HaCaT cells, THP-1 cells, Daudi cells, Ramos cells, Raji cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TAP1 Ready-To-Use IHC Kit. TAP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68412-1-Ig targets TAP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 Human
文献引用反应性human
免疫原 TAP1 fusion protein Ag21303 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
别名 ABC17, ABCB2, Antigen peptide transporter 1, APT1, D6S114E, FLJ26666, FLJ41500, Peptide supply factor 1, Peptide transporter PSF1, Peptide transporter TAP1, PSF 1, PSF1, RING4, TAP1, TAP1*0102N, TAP1N, Y3
计算分子量 81 kDa
观测分子量70 kDa
GenBank蛋白编号BC014081
基因名称 TAP1
Gene ID (NCBI) 6890
RRIDAB_3085128
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ03518
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TAP1, also known as ABCB2, PSF1 or RING4, is a member of the ATP-binding cassette (ABC) family of transmembrane transporters and is an essential component of the major histocompatability complex (MHC) class I antigen-presenting pathway. TAP is involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It also acts as a molecular scaffold for the final stage of MHC class I folding. Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1). Western blot analysis using this antibody detected a major band around 70 kDa.

实验方案

Product Specific Protocols
WB protocol for TAP1 antibody 68412-1-IgDownload protocol
IHC protocol for TAP1 antibody 68412-1-IgDownload protocol
IF protocol for TAP1 antibody 68412-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Agric Food Chem

Carnosic Acid: A Novel Selective Inhibitor of ERAP1 by Direct Binding and Its Modulation of Antigen Processing and Presentation

Authors - Jiaqi Wu
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