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TAP1 Monoclonal antibody, PBS Only

TAP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B8A7

Cat no : 68412-1-PBS

Print datasheet

Synonyms

ABC17, ABCB2, Antigen peptide transporter 1, APT1, D6S114E, FLJ26666, FLJ41500, Peptide supply factor 1, Peptide transporter PSF1, Peptide transporter TAP1, PSF 1, PSF1, RING4, TAP1, TAP1*0102N, TAP1N, Y3

验证数据展示

  • WB analysis using 68412-1-Ig (same clone as 68412-1-PBS)

    WB analysis using 68412-1-Ig (same clone as 68412-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68412-1-Ig (TAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68412-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 68412-1-Ig (same clone as 68412-1-PBS)

    IHC staining of human pancreas cancer using 68412-1-Ig (same clone as 68412-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68412-1-Ig (TAP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68412-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TAP1 antibody included
  • IHC staining of human pancreas cancer using 68412-1-Ig (same clone as 68412-1-PBS)

    IHC staining of human pancreas cancer using 68412-1-Ig (same clone as 68412-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68412-1-Ig (TAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68412-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TAP1 antibody included
  • IF Staining of HepG2 using 68412-1-Ig (same clone as 68412-1-PBS)

    IF Staining of HepG2 using 68412-1-Ig (same clone as 68412-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TAP1 antibody (68412-1-Ig, Clone: 1B8A7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68412-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TAP1 Ready-To-Use IHC Kit. TAP1 primary antibody included.

产品信息

68412-1-PBS targets TAP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen TAP1 fusion protein Ag21303 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
Synonyms ABC17, ABCB2, Antigen peptide transporter 1, APT1, D6S114E, FLJ26666, FLJ41500, Peptide supply factor 1, Peptide transporter PSF1, Peptide transporter TAP1, PSF 1, PSF1, RING4, TAP1, TAP1*0102N, TAP1N, Y3
Calculated Molecular Weight 81 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC014081
Gene Symbol TAP1
Gene ID (NCBI) 6890
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ03518
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TAP1, also known as ABCB2, PSF1 or RING4, is a member of the ATP-binding cassette (ABC) family of transmembrane transporters and is an essential component of the major histocompatability complex (MHC) class I antigen-presenting pathway. TAP is involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It also acts as a molecular scaffold for the final stage of MHC class I folding. Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1). Western blot analysis using this antibody detected a major band around 70 kDa.