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验证数据展示

  • WB analysis using 68577-1-Ig

    WB analysis using 68577-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68577-1-Ig (TAPBPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • FC experiment of A431 using 68577-1-Ig

    FC experiment of A431 using 68577-1-Ig

    1x10^6 A431 cells were intracellularly stained with 0.4 ug TAPBPL Monoclonal antibody (68577-1-Ig, Clone: 1D12C2) and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002) (red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

TAPBPL Monoclonal antibody

TAPBPL Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 68577-1-Ig
CloneNo. 1D12C2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

TAP-binding protein-like, Tapasin-related protein, TAPASIN-R, Tapasin-like, TAPASIN R

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inSCaBER cells, MCF-7 cells, HT-1376 cells, A431 cells, U-87 MG cells, Jurkat cells, human placenta tissue
Positive FC (Intra) detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68577-1-Ig targets TAPBPL in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen TAPBPL fusion protein Ag14371 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name TAP binding protein-like
Synonyms TAP-binding protein-like, Tapasin-related protein, TAPASIN-R, Tapasin-like, TAPASIN R
Calculated Molecular Weight 468 aa, 50 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberBC015017
Gene Symbol TAPBPL
Gene ID (NCBI) 55080
RRIDAB_3085277
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BX59
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TAPBPL, also named as TAPBP-R and TAPASIN-R, is a member of the variable-constant Ig superfamily that links MHC class I molecules to the transporter associated with antigen processing in the endoplasmic reticulum.

实验方案

Product Specific Protocols
WB protocol for TAPBPL antibody 68577-1-IgDownload protocol
FC protocol for TAPBPL antibody 68577-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols