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  • KD/KO Validated

WWTR1 Monoclonal antibody

WWTR1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse and More (1)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A12A10

Cat No : 66500-1-Ig

Print datasheet

Synonyms

TAZ, WWTR1



经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, HSC-T6 cells, MCF-7 cells, NIH/3T3 cells, MDA-MB-231 cells, HeLa cells, Jurkat cells, 4T1 cells, MDA-MB-453S cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66500-1-Ig targets WWTR1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity Human, rat, mouse
Cited Reactivityhuman, mouse, rat
Immunogen WWTR1 fusion protein Ag13330 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name WW domain containing transcription regulator 1
Synonyms TAZ, WWTR1
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC014052
Gene Symbol WWTR1
Gene ID (NCBI) 25937
RRIDAB_2881864
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9GZV5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma. TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.

实验方案

Product Specific Protocols
WB protocol for WWTR1 antibody 66500-1-IgDownload protocol
IHC protocol for WWTR1 antibody 66500-1-IgDownload protocol
IF protocol for WWTR1 antibody 66500-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Differ

Targeting USP10 induces degradation of oncogenic ANLN in esophageal squamous cell carcinoma

Authors - Yu-Fei Cao
humanWB

Nat Commun

LPA signaling acts as a cell-extrinsic mechanism to initiate cilia disassembly and promote neurogenesis.

Authors - Huai-Bin Hu
  • KD Validated
mouseWB,IHC,IF

Front Pharmacol

TAZ Induces Migration of Microglia and Promotes Neurological Recovery After Spinal Cord Injury.

Authors - Xuyang Hu
humanWB

Am J Transl Res

VASN promotes YAP/TAZ and EMT pathway in thyroid carcinogenesis in vitro.

Authors - Adheesh Bhandari
ratWB,IF

Toxicol Appl Pharmacol

Cysteine and glycine rich protein 2 exacerbates vascular fibrosis in pulmonary hypertension through the nuclear translocation of yes-associated protein and transcriptional coactivator with PDZ-binding motif

Authors - Xinghe Chen
humanWB,IHC

Stem Cells Dev

Reduced DAPK1 Expression Promotes Stem Cell-Like Characteristics of Prostate Cancer Cells by Activating ZEB1 via Hippo/YAP Signaling Pathway.

Authors - Shaojun Nong