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  • Featured Product
  • KD/KO Validated

WWTR1 Monoclonal antibody, PBS Only

WWTR1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

rat, mouse, human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A12A10

Cat no : 66500-1-PBS

Print datasheet

Synonyms

TAZ, WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif, 2A12A10

验证数据展示

  • WB analysis using 66500-1-Ig (same clone as 66500-1-PBS)

    WB analysis using 66500-1-Ig (same clone as 66500-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66500-1-Ig (TAZ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66500-1-Ig (same clone as 66500-1-PBS)

    WB analysis of HepG2 using 66500-1-Ig (same clone as 66500-1-PBS)

    WB result of TAZ antibody (66500-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAZ transfected HepG2 cells. This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66500-1-Ig (same clone as 66500-1-PBS)

    IHC staining of human liver cancer using 66500-1-Ig (same clone as 66500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66500-1-Ig (TAZ antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66500-1-Ig (same clone as 66500-1-PBS)

    IHC staining of human liver cancer using 66500-1-Ig (same clone as 66500-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66500-1-Ig (TAZ antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 66500-1-Ig (same clone as 66500-1-PBS)

    IF Staining of human liver cancer using 66500-1-Ig (same clone as 66500-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TAZ antibody (66500-1-Ig, Clone: 2A12A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66500-1-PBS in a different storage buffer formulation.

产品信息

66500-1-PBS targets WWTR1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity rat, mouse, human
Immunogen WWTR1 fusion protein Ag13330 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name WW domain containing transcription regulator 1
Synonyms TAZ, WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif, 2A12A10
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC014052
Gene Symbol WWTR1
Gene ID (NCBI) 25937
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9GZV5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma. TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.