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验证数据展示

  • WB analysis using 24667-1-AP (same clone as 24667-1-PBS)

    WB analysis using 24667-1-AP (same clone as 24667-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24667-1-AP (TBC1D4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

  • WB analysis using 24667-1-AP (same clone as 24667-1-PBS)

    WB analysis using 24667-1-AP (same clone as 24667-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24667-1-AP (TBC1D4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 24667-1-AP (same clone as 24667-1-PBS)

    IHC staining of human colon using 24667-1-AP (same clone as 24667-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 24667-1-AP (same clone as 24667-1-PBS)

    IHC staining of human colon using 24667-1-AP (same clone as 24667-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 24667-1-AP (same clone as 24667-1-PBS)

    IHC staining of mouse colon using 24667-1-AP (same clone as 24667-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24667-1-AP (TBC1D4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 24667-1-AP (same clone as 24667-1-PBS)

    IHC staining of mouse colon using 24667-1-AP (same clone as 24667-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24667-1-AP (TBC1D4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 24667-1-AP (same clone as 24667-1-PBS)

    IF Staining of HeLa using 24667-1-AP (same clone as 24667-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TBC1D4 antibody (24667-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

TBC1D4 Polyclonal antibody, PBS Only

TBC1D4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 24667-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

24667-1-PBS targets TBC1D4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag20327

Product name: Recombinant human TBC1D4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-200 aa of BC151239

Sequence: MEPPSCIQDEPFPHPLEPEPGVSAQPGPGKPSDKRFRLWYVGGSCLDHRTTLPMLPWLMAEIRRRSQKPEAGGCGAPAAREVILVLSAPFLRCVPAPGAGASGGTSPSATQPNPAVFIFEHKAQHISRFIHNSHDLTYFAYLIKAQPDDPESQMACHVFRATDPSQVPDVISSIRQLSKAAMKEDAKPSKDNEDAFYNSQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TBC1 domain family, member 4
别名
计算分子量 1299 aa, 147 kDa
观测分子量 160 kDa
GenBank蛋白编号BC151239
基因名称 TBC1D4
Gene ID (NCBI) 9882
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60343
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TBC1D4, also named as AS160, KIAA0603, may act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. The antibody recognizes all the isoform of TBC1D4.

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