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验证数据展示

  • WB analysis using 68063-1-Ig

    WB analysis using 68063-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68063-1-Ig (TBC1D4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

TBC1D4 Monoclonal antibody

TBC1D4 Monoclonal Antibody for WB, ELISA
Cat No. 68063-1-Ig

产品说明书

CloneNo. 1E2H2

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, ELISA

别名

Akt substrate of 160 kDa, AS160, DKFZp779C0666, KIAA0603, TBC1 domain family member 4, TBC1 domain family, member 4, TBC1D4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HCT 116 cells, LNCaP cells, Jurkat cells, K-562 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68063-1-Ig targets TBC1D4 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 TBC1D4 fusion protein Ag20341 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 TBC1 domain family, member 4
别名 Akt substrate of 160 kDa, AS160, DKFZp779C0666, KIAA0603, TBC1 domain family member 4, TBC1 domain family, member 4, TBC1D4
计算分子量 1299 aa, 147 kDa
观测分子量160 kDa
GenBank蛋白编号BC151239
基因名称 TBC1D4
Gene ID (NCBI) 9882
RRIDAB_2918804
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO60343
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBC1D4, also named as AS160, KIAA0603, may act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. The antibody recognizes all the isoform of TBC1D4.

实验方案

Product Specific Protocols
WB protocol for TBC1D4 antibody 68063-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols