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TBC1D4 Monoclonal antibody

TBC1D4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E2H2

Cat no : 68063-1-Ig

Print datasheet

Synonyms

Akt substrate of 160 kDa, AS160, DKFZp779C0666, KIAA0603, TBC1 domain family member 4, TBC1 domain family, member 4, TBC1D4

验证数据展示

  • WB analysis using 68063-1-Ig

    WB analysis using 68063-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68063-1-Ig (TBC1D4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HCT 116 cells, LNCaP cells, Jurkat cells, K-562 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68063-1-Ig targets TBC1D4 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen TBC1D4 fusion protein Ag20341 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name TBC1 domain family, member 4
Synonyms Akt substrate of 160 kDa, AS160, DKFZp779C0666, KIAA0603, TBC1 domain family member 4, TBC1 domain family, member 4, TBC1D4
Calculated Molecular Weight 1299 aa, 147 kDa
Observed Molecular Weight160 kDa
GenBank Accession NumberBC151239
Gene Symbol TBC1D4
Gene ID (NCBI) 9882
RRIDAB_2918804
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO60343
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBC1D4, also named as AS160, KIAA0603, may act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. The antibody recognizes all the isoform of TBC1D4.

实验方案

Product Specific Protocols
WB protocol for TBC1D4 antibody 68063-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols