验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 68063-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68063 targets TBC1D4 in IF/ICC applications and shows reactivity with Human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human |
| 免疫原 | TBC1D4 fusion protein Ag20341 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | TBC1 domain family, member 4 |
| 别名 | Akt substrate of 160 kDa, AS160, DKFZp779C0666, KIAA0603, TBC1 domain family member 4, TBC1 domain family, member 4, TBC1D4 |
| 计算分子量 | 1299 aa, 147 kDa |
| 观测分子量 | 160 kDa |
| GenBank蛋白编号 | BC151239 |
| 基因名称 | TBC1D4 |
| Gene ID (NCBI) | 9882 |
| RRID | AB_2934617 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O60343 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TBC1D4, also named as AS160, KIAA0603, may act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 TBC1D4 antibody CL488-68063 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |