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验证数据展示

  • WB analysis using 68371-1-Ig

    WB analysis using 68371-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68371-1-Ig (TBCC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • IF Staining of HeLa using 68371-1-Ig

    IF Staining of HeLa using 68371-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TBCC antibody (68371-1-Ig, Clone: 2B7D9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

TBCC Monoclonal antibody

TBCC Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68371-1-Ig

产品说明书

CloneNo. 2B7D9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Tubulin-specific chaperone C, Tubulin-folding cofactor C, CFC, 2B7D9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHT-1376 cells, Calu-3 cells, HeLa cells, MDA-MB-231 cells, MDA-MB-468 cells, HepG2 cells, HSC-T6 cells, 4T1 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68371-1-Ig targets TBCC in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TBCC fusion protein Ag8962 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tubulin folding cofactor C
别名 Tubulin-specific chaperone C, Tubulin-folding cofactor C, CFC, 2B7D9
计算分子量 346 aa, 39 kDa
观测分子量39 kDa
GenBank蛋白编号BC020170
基因名称 TBCC
Gene ID (NCBI) 6903
RRIDAB_3085091
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ15814
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBCC, also named CFC. Expressed in the retina. Expressed in the rod and cone photoreceptors, extending from the inner segments (IS), through the outer nuclear layer (ONL) and into the synapses in the outer plexiform layer (OPL). Strongly expressed to the photoreceptor connecting cilium at the tips of the IS (at protein level). Cofactor C was cytoplasmic in distribution (PMID: 12417528).

实验方案

Product Specific Protocols
WB protocol for TBCC antibody 68371-1-IgDownload protocol
IF protocol for TBCC antibody 68371-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols