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  • Featured Product
  • KD/KO Validated

TBK1 Monoclonal antibody, PBS Only

TBK1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D7B1

Cat no : 67211-1-PBS

Print datasheet

Synonyms

Serine/threonine-protein kinase TBK1, Serine/threonine protein kinase TBK1, NF-kappa-B-activating kinase, NF kappa B activating kinase, EC:2.7.11.1

验证数据展示

  • WB analysis using 67211-1-Ig (same clone as 67211-1-PBS)

    WB analysis using 67211-1-Ig (same clone as 67211-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67211-1-Ig (TBK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67211-1-PBS in a different storage buffer formulation.

  • WB analysis of U2OS using 67211-1-Ig (same clone as 67211-1-PBS)

    WB analysis of U2OS using 67211-1-Ig (same clone as 67211-1-PBS)

    U2OS (WT and TBK1 KO) lysates prepared with RIPA buffer, 50 μg protein loaded. 67211-1-Ig incubated at 1:5000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 67211-1-PBS in a different storage buffer formulation.

产品信息

67211-1-PBS targets TBK1 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, rat
Immunogen TBK1 fusion protein Ag28894 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name TANK-binding kinase 1
Synonyms Serine/threonine-protein kinase TBK1, Serine/threonine protein kinase TBK1, NF-kappa-B-activating kinase, NF kappa B activating kinase, EC:2.7.11.1
Observed Molecular Weight 84 kDa
GenBank Accession NumberBC034950
Gene Symbol TBK1
Gene ID (NCBI) 29110
RRIDAB_2882504
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UHD2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TBK1, also named as tumor necrosis factor (TNF) receptor-associated factor NF-kB activator (TANK)-binding kinase 1 (TBK1), NF-kB-activating kinase (NAK), T2K, is a multimeric kinase that modulates inflammation and autophagy. It is a ubiquitously expressed serine-threonine kinase belonging to the 'noncanonical IkB kinases' (IKKs) recognized for its critical role in regulating type I IFN production (PMID: 27211305). And TBK1 is an important player in yet another critical cellular function, autophagy. This antibody is specific for TBK1.