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TBX21,T-bet Recombinant antibody

TBX21,T-bet Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240849C1

Cat no : 83804-6-RR

Print datasheet

Synonyms

TBX21, TBX 21, T-box transcription factor TBX21, T-box protein 21, T bet

验证数据展示

  • WB analysis of CTLL-2 using 83804-6-RR

    WB analysis of CTLL-2 using 83804-6-RR

    CTLL-2 cells were subjected to SDS PAGE followed by western blot with 83804-6-RR (TBX21,T-bet antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83804-6-RR

    Affinity and Kinetic Characterization of 83804-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83804-6-RR against Human TBX21,T-bet were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inCTLL-2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83804-6-RR targets TBX21,T-bet in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen TBX21,T-bet fusion protein Ag4618 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name T-box 21
Synonyms TBX21, TBX 21, T-box transcription factor TBX21, T-box protein 21, T bet
Calculated Molecular Weight 535 aa, 58 kDa
Observed Molecular Weight60-65 kDa
GenBank Accession NumberBC039739
Gene Symbol TBX21/T-bet
Gene ID (NCBI) 30009
ENSEMBL Gene IDENSG00000073861
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UL17
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBX21 is a transcription factor that drives the Th1 immune response primarily through promoting expression of the IFN-γ gene. TBX21 initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. It has also been shown to play an influential role in inflammatory processes such as inflammatory bowel disease, autoimmune diseases such as rheumatoid arthritis, and immune-mediated conditions like asthma.

实验方案

Product Specific Protocols
WB protocol for TBX21,T-bet antibody 83804-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols