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TBXAS1 Monoclonal antibody

TBXAS1 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1F6G6

Cat no : 67371-1-Ig

Print datasheet

Synonyms

Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.5, EC:4.2.1.152, CYP5A1, CYP5

验证数据展示

  • WB analysis of human peripheral blood platelets using 67371-1-Ig

    WB analysis of human peripheral blood platelets using 67371-1-Ig

    Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67371-1-Ig (TBXAS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 67371-1-Ig

    WB analysis of A549 using 67371-1-Ig

    WB result of TBXAS1 antibody (67371-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBXAS1 transfected A549 cells.

  • IHC staining of human lung cancer using 67371-1-Ig

    IHC staining of human lung cancer using 67371-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67371-1-Ig (TBXAS1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 67371-1-Ig

    IHC staining of human lung cancer using 67371-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67371-1-Ig (TBXAS1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of A549 using 67371-1-Ig

    FC experiment of A549 using 67371-1-Ig

    1x10^6 A549 cells were intracellularly stained with 0.8 ug TBXAS1 Monoclonal antibody (67371-1-Ig, Clone:1F6G6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inhuman peripheral blood platelets, A549 cells, human placenta tissue, pig blood
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67371-1-Ig targets TBXAS1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, pig
Cited Reactivitymouse
Immunogen TBXAS1 fusion protein Ag29177 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name thromboxane A synthase 1 (platelet)
Synonyms Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.5, EC:4.2.1.152, CYP5A1, CYP5
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC014117
Gene Symbol TBXAS1
Gene ID (NCBI) 6916
RRIDAB_2918487
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP24557
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBXAS1 catalyzes the conversion of the prostaglandin endoperoxide into thromboxane A2 as a potent vasoconstrictor and inducer of platelet aggregation.It was identified as novel airway fibroblast-specific markers with integrin-8 (ITGA8) (PMID:20435685). It was observed in the epithelium.It is a monomer with the molecular weight of 60 KDa. It also has two variants with 60.5 kDa and 52.3 kDa (PMID:1730669). It also reavels a molecular weight of 55 kDa in human uterus (PMID:1386258).

实验方案

Product Specific Protocols
WB protocol for TBXAS1 antibody 67371-1-IgDownload protocol
IHC protocol for TBXAS1 antibody 67371-1-IgDownload protocol
FC protocol for TBXAS1 antibody 67371-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Am J Physiol Renal Physiol

Inhibition of COX-1 attenuates the formation of thromboxane A2 and ameliorates the acute decrease in glomerular filtration rate in endotoxemic mice.

Authors - Katharina Mederle
View Article