验证数据展示
经过测试的应用
| Positive WB detected in | human peripheral blood platelets, A549 cells, human placenta tissue, pig blood |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | A549 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
67371-1-Ig targets TBXAS1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, pig samples.
| Tested Applications | WB, IHC, FC (Intra), ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, pig |
| Cited Reactivity | mouse |
| Immunogen | TBXAS1 fusion protein Ag29177 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | thromboxane A synthase 1 (platelet) |
| Synonyms | Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.5, EC:4.2.1.152, CYP5A1, CYP5 |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC014117 |
| Gene Symbol | TBXAS1 |
| Gene ID (NCBI) | 6916 |
| RRID | AB_2918487 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P24557 |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TBXAS1 catalyzes the conversion of the prostaglandin endoperoxide into thromboxane A2 as a potent vasoconstrictor and inducer of platelet aggregation.It was identified as novel airway fibroblast-specific markers with integrin-8 (ITGA8) (PMID:20435685). It was observed in the epithelium.It is a monomer with the molecular weight of 60 KDa. It also has two variants with 60.5 kDa and 52.3 kDa (PMID:1730669). It also reavels a molecular weight of 55 kDa in human uterus (PMID:1386258).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TBXAS1 antibody 67371-1-Ig | Download protocol |
| IHC protocol for TBXAS1 antibody 67371-1-Ig | Download protocol |
| FC protocol for TBXAS1 antibody 67371-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
