• Featured Product
  • KD/KO验证

TBXAS1 Monoclonal antibody

TBXAS1 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 67371-1-Ig

产品说明书

CloneNo. 1F6G6

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, pig

应用

WB, IHC, FC (Intra), ELISA

Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.5, EC:4.2.1.152, CYP5A1, CYP5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inhuman peripheral blood platelets, A549 cells, human placenta tissue, pig blood
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67371-1-Ig targets TBXAS1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, pig samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, pig
文献引用反应性mouse
免疫原 TBXAS1 fusion protein Ag29177 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 thromboxane A synthase 1 (platelet)
别名 Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.5, EC:4.2.1.152, CYP5A1, CYP5
计算分子量 60 kDa
观测分子量 52 kDa
GenBank蛋白编号BC014117
基因名称 TBXAS1
Gene ID (NCBI) 6916
RRIDAB_2918487
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP24557
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBXAS1 catalyzes the conversion of the prostaglandin endoperoxide into thromboxane A2 as a potent vasoconstrictor and inducer of platelet aggregation.It was identified as novel airway fibroblast-specific markers with integrin-8 (ITGA8) (PMID:20435685). It was observed in the epithelium.It is a monomer with the molecular weight of 60 KDa. It also has two variants with 60.5 kDa and 52.3 kDa (PMID:1730669). It also reavels a molecular weight of 55 kDa in human uterus (PMID:1386258).

实验方案

Product Specific Protocols
WB protocol for TBXAS1 antibody 67371-1-IgDownload protocol
IHC protocol for TBXAS1 antibody 67371-1-IgDownload protocol
FC protocol for TBXAS1 antibody 67371-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Am J Physiol Renal Physiol

Inhibition of COX-1 attenuates the formation of thromboxane A2 and ameliorates the acute decrease in glomerular filtration rate in endotoxemic mice.

Authors - Katharina Mederle
Loading...