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验证数据展示

  • FC experiment of HepG2 using CL488-67523

    FC experiment of HepG2 using CL488-67523

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TCF12 (CL488-67523, Clone:1B8D4) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated TCF12 Monoclonal antibody

TCF12 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67523

产品说明书

CloneNo. 1B8D4

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

FC (Intra)

别名

TCF12, TCF12/HEB, transcription factor 12, Transcription factor HTF-4, TCF-12, 1B8D4, bHLHb20, Class B basic helix-loop-helix protein 20, DNA-binding protein HTF4, E box binding protein, E-box-binding protein, HEB, HsT17266, HTF4, TCF 12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TCF12 Ready-To-Use IHC Kit. TCF12 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67523-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67523 targets TCF12 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, mouse, rat
免疫原 TCF12 fusion protein Ag6287 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 transcription factor 12
别名 TCF12, TCF12/HEB, transcription factor 12, Transcription factor HTF-4, TCF-12, 1B8D4, bHLHb20, Class B basic helix-loop-helix protein 20, DNA-binding protein HTF4, E box binding protein, E-box-binding protein, HEB, HsT17266, HTF4, TCF 12
计算分子量 73 kDa
观测分子量 80 kDa
GenBank蛋白编号BC050556
基因名称 TCF12
Gene ID (NCBI) 6938
RRIDAB_2934546
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99081
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The product of TCF12 gene (TCF12, also called HTF4 or HEB) is a member of helix-loop-helix (HLH) protein family [PMID:22130667]. TCF12 is a transcriptional regulator that involved in the initiation of neuronal differentiation. It 's also known as E proteins, and activates transcription by binding to the E box (5'-CANNTG-3'), because of their direct DNA (E-box) binding ability [PMID: 19204326]. TCF12 was shown to suppress E-cadherin expression during the early stages of renal tubular epithelial cell dedifferentiation [PMID:17202424].

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 TCF12 antibody CL488-67523Download protocol
Standard Protocols
Click here to view our Standard Protocols