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验证数据展示

  • IF Staining of mouse skeletal muscle using CL488-22337

    IF Staining of mouse skeletal muscle using CL488-22337

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using CoraLite® Plus 488 TCF4 antibody (CL488-22337) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated TCF4 Polyclonal antibody

TCF4 Polyclonal Antibody for IF-P
Cat No. CL488-22337

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF-P

别名

bHLHb19, E2 2, ITF 2, ITF2, PTHS, SEF 2, SEF2, SEF2 1, SEF2 1A, SEF2 1B, SL3 3 enhancer factor 2, TCF 4, TCF4, transcription factor 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse skeletal muscle tissue
Planning an IHC experiment? We recommend our IHCeasy TCF4 Ready-To-Use IHC Kit. TCF4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 22337-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-22337 targets TCF4 in IF-P applications and shows reactivity with human, mouse samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse
免疫原 TCF4 fusion protein Ag17813 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transcription factor 4
别名 bHLHb19, E2 2, ITF 2, ITF2, PTHS, SEF 2, SEF2, SEF2 1, SEF2 1A, SEF2 1B, SL3 3 enhancer factor 2, TCF 4, TCF4, transcription factor 4
计算分子量 671 aa, 72 kDa
观测分子量 72 kDa
GenBank蛋白编号BC125084
基因名称 TCF4
Gene ID (NCBI) 6925
RRIDAB_3672742
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP15884
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transcription factor 4 (TCF4), also known as SEF2, ITF2, E2-2, and ME2, binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription By similarity. Human TCF4 mRNA expression is particularly high in the brain. Usage of numerous 5' exons of the human TCF4 gene potentially yields in TCF4 protein isoforms with 18 different N-termini. In addition, the diversity of isoforms is increased by alternative splicing of several internal exons. Some isoforms contain a bipartite nuclear localization signal and are exclusively nuclear, whereas distribution of other isoforms relies on heterodimerization partners. Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.TCF4 has several splicing isoforms that are expressed differentially in tissues and during cancer progression(15547706, 12796377). The scopel of molecular weight of several isoforms is about 60-90 kDa (21789225).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TCF4 antibody CL488-22337Download protocol
Standard Protocols
Click here to view our Standard Protocols