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TCF7L1 Recombinant antibody, PBS Only (Capture)

TCF7L1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240544F1

Cat no : 83588-3-PBS

Print datasheet

Synonyms

Transcription factor 7-like 1, TCF7L2, TCF4, TCF-3, TCF3

验证数据展示

  • WB analysis using 83588-3-RR (same clone as 83588-3-PBS)

    WB analysis using 83588-3-RR (same clone as 83588-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83588-3-RR (TCF7L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83588-3-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 83588-3-RR (same clone as 83588-3-PBS)

    FC experiment of A549 using 83588-3-RR (same clone as 83588-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug TCF7L1 Recombinant antibody (83588-3-RR, Clone:240544F1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83588-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00573-1

    Cytometric bead array standard curve of MP00573-1

    Cytometric bead array standard curve of MP00573-1, TCF7L1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83588-3-PBS. Detection antibody: 83588-2-PBS. Standard: Ag34253. Range: 1.25-80 ng/mL.

  • Cytometric bead array standard curve of MP00573-2

    Cytometric bead array standard curve of MP00573-2

    Cytometric bead array standard curve of MP00573-2, TCF7L1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83588-3-PBS. Detection antibody: 83588-1-PBS. Standard: Ag34253. Range: 1.25-80 ng/mL.

  • Affinity and Kinetic Characterization of 83588-3-RR

    Affinity and Kinetic Characterization of 83588-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83588-3-RR against Human TCF7L1 were performed. The affinity constant is below 1 pM.

产品信息

83588-3-PBS targets TCF7L1 as part of a matched antibody pair:

MP00573-1: 83588-3-PBS capture and 83588-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen TCF7L1 fusion protein Ag34253 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name transcription factor 7-like 1 (T-cell specific, HMG-box)
Synonyms Transcription factor 7-like 1, TCF7L2, TCF4, TCF-3, TCF3
Calculated Molecular Weight 63 kDa
GenBank Accession NumberBC058894
Gene Symbol TCF7L1
Gene ID (NCBI) 83439
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9HCS4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C