验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HeLa cells, HEK-293 cells, NCCIT cells, NIH/3T3 cells |
Positive FC (Intra) detected in | A549 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83588-3-RR targets TCF7L1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, FC (Intra), ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | TCF7L1 fusion protein Ag34253 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | transcription factor 7-like 1 (T-cell specific, HMG-box) |
Synonyms | Transcription factor 7-like 1, TCF7L2, TCF4, TCF-3, TCF3 |
Calculated Molecular Weight | 63 kDa |
Observed Molecular Weight | 63 kDa, 75 kDa |
GenBank Accession Number | BC058894 |
Gene Symbol | TCF7L1 |
Gene ID (NCBI) | 83439 |
RRID | AB_3671202 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9HCS4 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TCF7L1 is one of TCF/LEF transcription factors that are mediators of the Wnt signaling pathway and are antagonized by the TGF-beta signaling pathway. It binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. It's necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis
实验方案
Product Specific Protocols | |
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WB protocol for TCF7L1 antibody 83588-3-RR | Download protocol |
FC protocol for TCF7L1 antibody 83588-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |