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验证数据展示

  • WB analysis using 83588-3-RR

    WB analysis using 83588-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83588-3-RR (TCF7L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of A549 using 83588-3-RR

    FC experiment of A549 using 83588-3-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug TCF7L1 Recombinant antibody (83588-3-RR, Clone:240544F1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

TCF7L1 Recombinant antibody

TCF7L1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83588-3-RR

产品说明书

CloneNo. 240544F1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

Transcription factor 7-like 1, TCF7L2, TCF4, TCF-3, TCF3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HEK-293 cells, NCCIT cells, NIH/3T3 cells
Positive FC (Intra) detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83588-3-RR targets TCF7L1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 TCF7L1 fusion protein Ag34253 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transcription factor 7-like 1 (T-cell specific, HMG-box)
别名 Transcription factor 7-like 1, TCF7L2, TCF4, TCF-3, TCF3
计算分子量 63 kDa
观测分子量63 kDa, 75 kDa
GenBank蛋白编号BC058894
基因名称 TCF7L1
Gene ID (NCBI) 83439
RRIDAB_3671202
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9HCS4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TCF7L1 is one of TCF/LEF transcription factors that are mediators of the Wnt signaling pathway and are antagonized by the TGF-beta signaling pathway. It binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. It's necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis

实验方案

Product Specific Protocols
WB protocol for TCF7L1 antibody 83588-3-RRDownload protocol
FC protocol for TCF7L1 antibody 83588-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols