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CoraLite® Plus 488-conjugated TCF7L1/TCF7L2 Monoclonal antibody

TCF7L1/TCF7L2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B2D12

Cat no : CL488-67652

Print datasheet

Synonyms

TCF7L1, TCF7L2, TCF4, TCF-3, TCF3

验证数据展示

  • FC experiment of A431 using CL488-67652

    FC experiment of A431 using CL488-67652

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TCF7L1/TCF7L2 (CL488-67652, Clone:2B2D12) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 67652-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67652 targets TCF7L1/TCF7L2 in FC (Intra) applications and shows reactivity with Human, Mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, Mouse
Immunogen TCF7L1/TCF7L2 fusion protein Ag28954 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name transcription factor 7-like 1 (T-cell specific, HMG-box)
Synonyms TCF7L1, TCF7L2, TCF4, TCF-3, TCF3
Calculated Molecular Weight 63 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC058894
Gene Symbol TCF7L1
Gene ID (NCBI) 83439
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9HCS4
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TCF7L1 is one of TCF/LEF transcription factors that are mediators of the Wnt signaling pathway and are antagonized by the TGF-beta signaling pathway. It binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. It's necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis. This antibody raised against 1-200aa of human TCF7L1 can also react with TCF7L2.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 TCF7L1/TCF7L2 antibody CL488-67652Download protocol
Standard Protocols
Click here to view our Standard Protocols