CoraLite® Plus 488-conjugated TDG Recombinant antibody
TDG Uni-rAbTM Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
240715E12
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy TDG Ready-To-Use IHC Kit. TDG primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83780-3-PBS
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of CL488-83780-3 is TDG Fusion Protein expressed in E. coli.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | human |
Immunogen | TDG fusion protein Ag4190 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | thymine-DNA glycosylase |
Synonyms | Thymine-DNA glycosylase, thymine DNA glycosylase, hTDG, G/T mismatch-specific thymine DNA glycosylase, EC:3.2.2.29 |
Calculated Molecular Weight | 410 aa, 46 kDa |
GenBank Accession Number | BC037557 |
Gene Symbol | TDG |
Gene ID (NCBI) | 6996 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q13569 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TDG belongs to the TDG/mug DNA glycosylase family. TDG corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. RNF4 interacts with and requires the base excision repair enzymes TDG and APE1 for active demethylation (PMID:20696907). TDG is modified by SUMO-1 and SUMO-2/3.The molecular weight of non-modified TDG is 46 kDa and modified TDG is 55-60 kDa.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 TDG antibody CL488-83780-3 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |