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TDRKH Monoclonal antibody, PBS Only

TDRKH Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B1B12

Cat no : 66845-1-PBS

Print datasheet

Synonyms

TDRD2, TDRKH, tudor and KH domain containing

验证数据展示

  • WB analysis using 66845-1-Ig (same clone as 66845-1-PBS)

    WB analysis using 66845-1-Ig (same clone as 66845-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66845-1-Ig (same clone as 66845-1-PBS)

    IF Staining of HepG2 using 66845-1-Ig (same clone as 66845-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66845-1-Ig (TDRKH antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

  • WB analysis using 66845-1-Ig (same clone as 66845-1-PBS)

    WB analysis using 66845-1-Ig (same clone as 66845-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

  • WB analysis using 66845-1-Ig (same clone as 66845-1-PBS)

    WB analysis using 66845-1-Ig (same clone as 66845-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 66845-1-Ig (same clone as 66845-1-PBS)

    IHC staining of mouse testis using 66845-1-Ig (same clone as 66845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66845-1-Ig (TDRKH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 66845-1-Ig (same clone as 66845-1-PBS)

    IHC staining of mouse testis using 66845-1-Ig (same clone as 66845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66845-1-Ig (TDRKH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66845-1-PBS in a different storage buffer formulation.

产品信息

66845-1-PBS targets TDRKH in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat, pig
Immunogen TDRKH fusion protein Ag4720 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tudor and KH domain containing
Synonyms TDRD2, TDRKH, tudor and KH domain containing
Calculated Molecular Weight 606 aa, 67 kDa
Observed Molecular Weight 67-70 kDa
GenBank Accession NumberBC032690
Gene Symbol TDRKH
Gene ID (NCBI) 11022
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y2W6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tudor and KH domain-containing protein(TDRKH)Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. Both KH and tudor domains are involved in binding RNA or single-strand DNA. Tudor also is a germ cell-specific protein with multiple Tudor domains and is involved in germ plasm formation and germ cell specification. TDRKH is one Tudor protein with a single Tudor domain.