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TDRKH Monoclonal antibody

TDRKH Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B1B12

Cat no : 66845-1-Ig

Print datasheet

Synonyms

TDRD2, TDRKH, tudor and KH domain containing

验证数据展示

  • WB analysis using 66845-1-Ig

    WB analysis using 66845-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IF Staining of HepG2 using 66845-1-Ig

    IF Staining of HepG2 using 66845-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66845-1-Ig (TDRKH antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis using 66845-1-Ig

    WB analysis using 66845-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 66845-1-Ig

    WB analysis using 66845-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66845-1-Ig (TDRKH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 66845-1-Ig

    IHC staining of mouse testis using 66845-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66845-1-Ig (TDRKH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 66845-1-Ig

    IHC staining of mouse testis using 66845-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66845-1-Ig (TDRKH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inrat brain tissue, JAR cells, NCCIT cells, LNCaP cells, pig brain tissue, mouse brain tissue, rabbit brain tissue, rat testis tissue, mouse testis tissue, Rat brain
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66845-1-Ig targets TDRKH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat, pig
Immunogen TDRKH fusion protein Ag4720 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tudor and KH domain containing
Synonyms TDRD2, TDRKH, tudor and KH domain containing
Calculated Molecular Weight 606 aa, 67 kDa
Observed Molecular Weight 67-70 kDa
GenBank Accession NumberBC032690
Gene Symbol TDRKH
Gene ID (NCBI) 11022
RRIDAB_2882185
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y2W6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tudor and KH domain-containing protein(TDRKH)Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. Both KH and tudor domains are involved in binding RNA or single-strand DNA. Tudor also is a germ cell-specific protein with multiple Tudor domains and is involved in germ plasm formation and germ cell specification. TDRKH is one Tudor protein with a single Tudor domain.

实验方案

Product Specific Protocols
WB protocol for TDRKH antibody 66845-1-IgDownload protocol
IHC protocol for TDRKH antibody 66845-1-IgDownload protocol
IF protocol for TDRKH antibody 66845-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols