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验证数据展示

  • FC experiment of MCF-7 using CL488-66845

    FC experiment of MCF-7 using CL488-66845

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TDRKH (CL488-66845, Clone:2B1B12) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL488-66845

    IF Staining of HepG2 using CL488-66845

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 TDRKH antibody (CL488-66845, Clone: 2B1B12 ) at dilution of 1:800.

CoraLite® Plus 488-conjugated TDRKH Monoclonal antibody

TDRKH Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66845

产品说明书

CloneNo. 2B1B12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF/ICC, FC (Intra)

别名

TDRKH specific, Tudor domain-containing protein 2, Tudor and KH domain-containing protein, tudor and KH domain containing, TDRD2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 66845-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66845 targets TDRKH in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原 TDRKH fusion protein Ag4720 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tudor and KH domain containing
别名 TDRKH specific, Tudor domain-containing protein 2, Tudor and KH domain-containing protein, tudor and KH domain containing, TDRD2
计算分子量 606 aa, 67 kDa
观测分子量 67-70 kDa
GenBank蛋白编号BC032690
基因名称 TDRKH
Gene ID (NCBI) 11022
RRIDAB_2923759
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y2W6
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tudor and KH domain-containing protein(TDRKH)Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. Both KH and tudor domains are involved in binding RNA or single-strand DNA. Tudor also is a germ cell-specific protein with multiple Tudor domains and is involved in germ plasm formation and germ cell specification. TDRKH is one Tudor protein with a single Tudor domain.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TDRKH antibody CL488-66845Download protocol
Standard Protocols
Click here to view our Standard Protocols