验证数据展示
经过测试的应用
| Positive IF/ICC detected in | U2OS cells |
| Positive FC (Intra) detected in | MCF-7 cells |
For other applications, we recommend the unconjugated version of this antibody, 66845-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL555-66845 targets TDRKH in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | human |
| 免疫原 | TDRKH fusion protein Ag4720 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | tudor and KH domain containing |
| 别名 | TDRKH specific, Tudor domain-containing protein 2, Tudor and KH domain-containing protein, tudor and KH domain containing, TDRD2 |
| 计算分子量 | 606 aa, 67 kDa |
| 观测分子量 | 67-70 kDa |
| GenBank蛋白编号 | BC032690 |
| 基因名称 | TDRKH |
| Gene ID (NCBI) | 11022 |
| RRID | AB_2934679 |
| 偶联类型 | CoraLite®555 Fluorescent Dye |
| 最大激发/发射波长 | 557 nm / 570 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9Y2W6 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tudor and KH domain-containing protein(TDRKH)Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. Both KH and tudor domains are involved in binding RNA or single-strand DNA. Tudor also is a germ cell-specific protein with multiple Tudor domains and is involved in germ plasm formation and germ cell specification. TDRKH is one Tudor protein with a single Tudor domain.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL555 TDRKH antibody CL555-66845 | Download protocol |
| FC protocol for CL555 TDRKH antibody CL555-66845 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |