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TERF2 Monoclonal antibody, PBS Only

TERF2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5B1E1

Cat no : 66893-1-PBS

Print datasheet

Synonyms

Telomeric DNA binding protein, TERF2, TRBF2, TRF2, TTAGGG repeat binding factor 2

验证数据展示

  • WB analysis of MCF-7 using 66893-1-Ig (same clone as 66893-1-PBS)

    WB analysis of MCF-7 using 66893-1-Ig (same clone as 66893-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 66893-1-Ig (same clone as 66893-1-PBS)

    IHC staining of human gliomas using 66893-1-Ig (same clone as 66893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 66893-1-Ig (same clone as 66893-1-PBS)

    IHC staining of human gliomas using 66893-1-Ig (same clone as 66893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66893-1-Ig (TERF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66893-1-Ig (same clone as 66893-1-PBS)

    WB analysis of HEK-293 using 66893-1-Ig (same clone as 66893-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 66893-1-Ig (same clone as 66893-1-PBS)

    WB analysis of Jurkat using 66893-1-Ig (same clone as 66893-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.

  • WB analysis of 4T1 using 66893-1-Ig (same clone as 66893-1-PBS)

    WB analysis of 4T1 using 66893-1-Ig (same clone as 66893-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 66893-1-Ig (TERF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66893-1-PBS in a different storage buffer formulation.

产品信息

66893-1-PBS targets TERF2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen TERF2 fusion protein Ag28513 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name telomeric repeat binding factor 2
Synonyms Telomeric DNA binding protein, TERF2, TRBF2, TRF2, TTAGGG repeat binding factor 2
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 60-65.32-35 kDa
GenBank Accession NumberBC024890
Gene Symbol TERF2
Gene ID (NCBI) 7014
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15554
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TERF2, also named as TRF2 and Telomeric repeat-binding factor 2, is a 542 amino acid protein, which contains 1 HTH myb-type DNA-binding domain and localizes in the Nucleus. TRF2 binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. TERF2 is a component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. TERF2 together with DCLRE1B/Apollo, is required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B), which is needed for telomere replication during fork passage and prevent aberrant telomere topology. TERF2 recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length. TERF2 exists some isoforms with MV 60 kDa and 32 kDa.