验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, MCF-7 cells, HT-29 cells, K-562 cells, HEK-293 cells, Daudi cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
| Positive IHC detected in | human gliomas tissue, rat brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66893-1-Ig targets TERF2 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, ChIP |
| 经测试反应性 | Human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | TERF2 fusion protein Ag28513 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | telomeric repeat binding factor 2 |
| 别名 | Telomeric DNA binding protein, TERF2, TRBF2, TRF2, TTAGGG repeat binding factor 2 |
| 计算分子量 | 56 kDa |
| 观测分子量 | 60-65.32-35 kDa |
| GenBank蛋白编号 | BC024890 |
| 基因名称 | TERF2 |
| Gene ID (NCBI) | 7014 |
| RRID | AB_2882222 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q15554 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TERF2, also named as TRF2 and Telomeric repeat-binding factor 2, is a 542 amino acid protein, which contains 1 HTH myb-type DNA-binding domain and localizes in the Nucleus. TRF2 binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. TERF2 is a component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. TERF2 together with DCLRE1B/Apollo, is required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B), which is needed for telomere replication during fork passage and prevent aberrant telomere topology. TERF2 recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length. TERF2 exists some isoforms with MV 60 kDa and 32 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TERF2 antibody 66893-1-Ig | Download protocol |
| IHC protocol for TERF2 antibody 66893-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Regulation of telomere homeostasis and genomic stability in cancer by N 6-adenosine methylation (m6A). | ||
Front Pharmacol Curcumol Suppresses CCF-Mediated Hepatocyte Senescence Through Blocking LC3B-Lamin B1 Interaction in Alcoholic Fatty Liver Disease. | ||
Nat Commun Transcription stress at telomeres leads to cytosolic DNA release and paracrine senescence | ||
Acta Biochim Biophys Sin (Shanghai) miR-29b-1-5p exacerbates myocardial injury induced by sepsis in a mouse model by targeting TERF2 | ||
