• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of U2OS using CL488-66893

    FC experiment of U2OS using CL488-66893

    1x10^6 U2OS cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Terf2 Monoclonal Antibody (CL488-66893, Clone:5B1E1)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated TERF2 Monoclonal antibody

TERF2 Monoclonal Antibody for FC (Intra)
Cat No. CL488-66893

产品说明书

CloneNo. 5B1E1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

TTAGGG repeat-binding factor 2, TRF2, TRBF2, Telomeric repeat-binding factor 2, Telomeric DNA-binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive FC (Intra) detected inU2OS cells
For other applications, we recommend the unconjugated version of this antibody, 66893-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66893 targets TERF2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 TERF2 fusion protein Ag28513 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 telomeric repeat binding factor 2
别名 TTAGGG repeat-binding factor 2, TRF2, TRBF2, Telomeric repeat-binding factor 2, Telomeric DNA-binding protein
计算分子量 56 kDa
观测分子量 60-65.32-35 kDa
GenBank蛋白编号BC024890
基因名称 TERF2
Gene ID (NCBI) 7014
RRIDAB_3672927
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ15554
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TERF2, also named as TRF2 and Telomeric repeat-binding factor 2, is a 542 amino acid protein, which contains 1 HTH myb-type DNA-binding domain and localizes in the Nucleus. TRF2 binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. TERF2 is a component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. TERF2 together with DCLRE1B/Apollo, is required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B), which is needed for telomere replication during fork passage and prevent aberrant telomere topology. TERF2 recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length. TERF2 exists some isoforms with MV 60 kDa and 32 kDa.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 TERF2 antibody CL488-66893Download protocol
Standard Protocols
Click here to view our Standard Protocols