验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, A431 cells, HeLa cells, HT-1080 cells, human placenta tissue, K-562 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
14595-1-AP targets TERF2IP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | TERF2IP fusion protein Ag6130 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | telomeric repeat binding factor 2, interacting protein |
| 别名 | Telomeric repeat-binding factor 2-interacting protein 1, RAP1 homolog, PP8000, hRap1, DRIP5 |
| 计算分子量 | 44 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC005841 |
| 基因名称 | TERF2IP |
| Gene ID (NCBI) | 54386 |
| RRID | AB_10642697 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NYB0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TERF2IP, also named as DRIP5, RAP1, is a 399 amino acid protein, which belongs to the RAP1 family. TERF2IP associates with chromosomes, both at telomeres and in extratelomeric sites. TERF2IP acts both as a regulator of telomere function and as a transcription regulator. TERF2IP is involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. The calculated molecular weight of TERF2IP is 44 kDa, but modified TERF2IP, is about 60 kDa. (PMID: 15838871)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TERF2IP antibody 14595-1-AP | Download protocol |
| IHC protocol for TERF2IP antibody 14595-1-AP | Download protocol |
| IF protocol for TERF2IP antibody 14595-1-AP | Download protocol |
| IP protocol for TERF2IP antibody 14595-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Preferential extension of short telomeres induced by low extracellular pH. | ||
Exp Cell Res C9ORF78 partially localizes to centromeres and plays a role in chromosome segregation. | ||
J Transl Med A multi-dimensional approach to unravel the intricacies of lactylation related signature for prognostic and therapeutic insight in colorectal cancer |
