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验证数据展示

  • IF Staining of HeLa using CL488-14595

    IF Staining of HeLa using CL488-14595

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 TERF2IP antibody (CL488-14595) at dilution of 1:200, CL594-phalloidin (red).

CoraLite® Plus 488-conjugated TERF2IP Polyclonal antibody

TERF2IP Polyclonal Antibody for IF/ICC
Cat No. CL488-14595

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

DRIP5, hRap1, RAP1 homolog, TERF2IP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TERF2IP Ready-To-Use IHC Kit. TERF2IP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14595-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14595 targets TERF2IP in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 TERF2IP fusion protein Ag6130 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 telomeric repeat binding factor 2, interacting protein
别名 DRIP5, hRap1, RAP1 homolog, TERF2IP
计算分子量 44 kDa
观测分子量 60 kDa
GenBank蛋白编号BC005841
基因名称 TERF2IP
Gene ID (NCBI) 54386
RRIDAB_3083960
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen Affinity Purified
UNIPROT IDQ9NYB0
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TERF2IP, also named as DRIP5, RAP1, is a 399 amino acid protein, which belongs to the RAP1 family. TERF2IP associates with chromosomes, both at telomeres and in extratelomeric sites. TERF2IP acts both as a regulator of telomere function and as a transcription regulator. TERF2IP is involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. The calculated molecular weight of TERF2IP is 44 kDa, but modified TERF2IP, is about 60 kDa. (PMID: 15838871)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TERF2IP antibody CL488-14595Download protocol
Standard Protocols
Click here to view our Standard Protocols