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验证数据展示

  • FC experiment of HeLa using CL488-21207

    FC experiment of HeLa using CL488-21207

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TET2 (CL488-21207) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated TET2 Polyclonal antibody

TET2 Polyclonal Antibody for FC (Intra)
Cat No. CL488-21207

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

FC (Intra)

别名

Nbla00191, Methylcytosine dioxygenase TET2, KIAA1546, EC:1.14.11.80

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells, RAW 264.7 cells
For other applications, we recommend the unconjugated version of this antibody, 21207-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21207 targets TET2 in FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 TET2 fusion protein Ag15583 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tet oncogene family member 2
别名 Nbla00191, Methylcytosine dioxygenase TET2, KIAA1546, EC:1.14.11.80
计算分子量 2002 aa, 224 kDa
观测分子量 130 kDa, 133 kDa, 224 kDa
GenBank蛋白编号BC150180
基因名称 TET2
Gene ID (NCBI) 54790
RRIDAB_2919179
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6N021
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TET2 is an enzyme which converts 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC) in DNA and is important for normal myelopoiesis. TET2 mutations are frequently observed in myeloid malignancies, including myeloproliferative neoplasms. TET2 is broadly expressed in a variety of tissues. In healthy tissues, TET2 expression was shown to be elevated in hematopoietic cells with highest expression in granulocytes. Three isoforms of TET2 exists due to the alternative splicing. 220-250 kDa bands represent the full-length isoform; 130-133 kDa bands represent the nuclear isoforms of TET2. (PMID: 24615693) This antibody recognizes all three isoforms of TET2.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 TET2 antibody CL488-21207Download protocol
Standard Protocols
Click here to view our Standard Protocols