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验证数据展示

  • FC experiment of HeLa using CL488-21207

    FC experiment of HeLa using CL488-21207

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TET2 (CL488-21207) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TET2 Polyclonal antibody

TET2 Polyclonal Antibody for FC (Intra)
Cat No. CL488-21207

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Synonyms

Nbla00191, Methylcytosine dioxygenase TET2, KIAA1546, EC:1.14.11.80

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells, RAW 264.7 cells
For other applications, we recommend the unconjugated version of this antibody, 21207-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21207 targets TET2 in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen TET2 fusion protein Ag15583 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tet oncogene family member 2
Synonyms Nbla00191, Methylcytosine dioxygenase TET2, KIAA1546, EC:1.14.11.80
Calculated Molecular Weight 2002 aa, 224 kDa
Observed Molecular Weight 130 kDa, 133 kDa, 224 kDa
GenBank Accession NumberBC150180
Gene Symbol TET2
Gene ID (NCBI) 54790
RRIDAB_2919179
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6N021
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TET2 is an enzyme which converts 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC) in DNA and is important for normal myelopoiesis. TET2 mutations are frequently observed in myeloid malignancies, including myeloproliferative neoplasms. TET2 is broadly expressed in a variety of tissues. In healthy tissues, TET2 expression was shown to be elevated in hematopoietic cells with highest expression in granulocytes. Three isoforms of TET2 exists due to the alternative splicing. 220-250 kDa bands represent the full-length isoform; 130-133 kDa bands represent the nuclear isoforms of TET2. (PMID: 24615693) This antibody recognizes all three isoforms of TET2.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 TET2 antibody CL488-21207Download protocol
Standard Protocols
Click here to view our Standard Protocols