验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse testis tissue, mouse brain tissue, HeLa cells, Neuro-2a cells |
| Positive IHC detected in | mouse testis tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25858-1-AP targets TEX264 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | TEX264 fusion protein Ag23027 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | testis expressed 264 |
| 别名 | ZSIG11, UNQ337/PRO536, TEX 264, Testis-expressed protein 264, TEG 264 |
| 计算分子量 | 313 aa, 34 kDa |
| 观测分子量 | 37 kDa |
| GenBank蛋白编号 | BC008742 |
| 基因名称 | TEX264 |
| Gene ID (NCBI) | 51368 |
| RRID | AB_2880272 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y6I9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TEX264 (testes expressed gene 264) is a single-pass transmembrane protein, consisting of an N-terminal hydrophobic region, a gyrase inhibitory (GyrI)-like domain, and a loosely structured C terminus. TEX264 was first identified as an endoplasmic reticulum (ER)-resident Atg8-family-binding protein that mediates the degradation of portions of the ER during starvation (i.e., reticulophagy). TEX264 was identified as a cofactor of VCP/p97 ATPase that promotes the repair of covalently trapped TOP1 (DNA topoisomerase 1)-DNA crosslinks.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TEX264 antibody 25858-1-AP | Download protocol |
| IHC protocol for TEX264 antibody 25858-1-AP | Download protocol |
| IF protocol for TEX264 antibody 25858-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun AMFR-mediated Flavivirus NS2A ubiquitination subverts ER-phagy to augment viral pathogenicity | ||
ACS Chem Neurosci Anti-Inflammatory Anthranilate Analogue Enhances Autophagy through mTOR and Promotes ER-Turnover through TEX264 during Alzheimer-Associated Neuroinflammation.
| ||
Biomedicines Enhancement of TEX264-Mediated ER-Phagy Contributes to the Therapeutic Effect of Glycycoumarin against APA Hepatotoxicity in Mice. | ||
Mol Biol Cell Lysosome Docking to WIPI1 Rings and ER-connected Phagophores Occurs During DNAJB12 and GABARAP-Dependent Selective Autophagy of Misfolded P23H-Rhodopsin. | ||
