• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TEX264 Polyclonal antibody

TEX264 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-25858

Print datasheet

Synonyms

SIG11, testis expressed 264, TEX264, ZSIG11

验证数据展示

  • FC experiment of U-251 using CL488-25858

    FC experiment of U-251 using CL488-25858

    1x10^6 U-251 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Tex264 Polyclonal Antibody (CL488-25858)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of U-251 using CL488-25858

    IF Staining of U-251 using CL488-25858

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using CoraLite® Plus 488 TEX264 antibody (CL488-25858) at dilution of 1:200.

经过测试的应用

Positive IF detected inU-251 cells
Positive FC detected inU-251 cells
For other applications, we recommend the unconjugated version of this antibody, 25858-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-25858 targets TEX264 in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen TEX264 fusion protein Ag23027 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name testis expressed 264
Synonyms SIG11, testis expressed 264, TEX264, ZSIG11
Calculated Molecular Weight 313 aa, 34 kDa
Observed Molecular Weight37 kDa
GenBank Accession NumberBC008742
Gene Symbol TEX264
Gene ID (NCBI) 51368
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6I9
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TEX264 (testes expressed gene 264) is a single-pass transmembrane protein, consisting of an N-terminal hydrophobic region, a gyrase inhibitory (GyrI)-like domain, and a loosely structured C terminus. TEX264 was first identified as an endoplasmic reticulum (ER)-resident Atg8-family-binding protein that mediates the degradation of portions of the ER during starvation (i.e., reticulophagy). TEX264 was identified as a cofactor of VCP/p97 ATPase that promotes the repair of covalently trapped TOP1 (DNA topoisomerase 1)-DNA crosslinks.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TEX264 antibody CL488-25858Download protocol
FC protocol for CL Plus 488 TEX264 antibody CL488-25858Download protocol
Standard Protocols
Click here to view our Standard Protocols