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验证数据展示

  • FC experiment of U-251 using CL488-25858

    FC experiment of U-251 using CL488-25858

    1x10^6 U-251 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Tex264 Polyclonal Antibody (CL488-25858)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of U-251 using CL488-25858

    IF Staining of U-251 using CL488-25858

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using CoraLite® Plus 488 TEX264 antibody (CL488-25858) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated TEX264 Polyclonal antibody

TEX264 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-25858

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

ZSIG11, UNQ337/PRO536, TEX 264, Testis-expressed protein 264, TEG 264

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inU-251 cells
Positive FC (Intra) detected inU-251 cells
For other applications, we recommend the unconjugated version of this antibody, 25858-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-25858 targets TEX264 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 TEX264 fusion protein Ag23027 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 testis expressed 264
别名 ZSIG11, UNQ337/PRO536, TEX 264, Testis-expressed protein 264, TEG 264
计算分子量 313 aa, 34 kDa
观测分子量37 kDa
GenBank蛋白编号BC008742
基因名称 TEX264
Gene ID (NCBI) 51368
RRIDAB_3672795
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6I9
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TEX264 (testes expressed gene 264) is a single-pass transmembrane protein, consisting of an N-terminal hydrophobic region, a gyrase inhibitory (GyrI)-like domain, and a loosely structured C terminus. TEX264 was first identified as an endoplasmic reticulum (ER)-resident Atg8-family-binding protein that mediates the degradation of portions of the ER during starvation (i.e., reticulophagy). TEX264 was identified as a cofactor of VCP/p97 ATPase that promotes the repair of covalently trapped TOP1 (DNA topoisomerase 1)-DNA crosslinks.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TEX264 antibody CL488-25858Download protocol
FC protocol for CL Plus 488 TEX264 antibody CL488-25858Download protocol
Standard Protocols
Click here to view our Standard Protocols