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  • KD/KO Validated

TFAP2C Polyclonal antibody

TFAP2C Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14572-1-AP

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Synonyms

ap 2gamma, AP2 GAMMA, ERF1, hAP 2g, TFAP2C, TFAP2G, Transcription factor ERF 1



经过测试的应用

Positive WB detected inHeLa cells, human placenta tissue, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TFAP2C Ready-To-Use IHC Kit. TFAP2C primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14572-1-AP targets TFAP2C in WB, IHC, IP, chIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, chIP
Tested Reactivity human
Cited Reactivityhuman
Immunogen TFAP2C fusion protein Ag5648 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma)
Synonyms ap 2gamma, AP2 GAMMA, ERF1, hAP 2g, TFAP2C, TFAP2G, Transcription factor ERF 1
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC035664
Gene Symbol TFAP2C
Gene ID (NCBI) 7022
RRIDAB_2271424
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92754
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TFAP2C antibody 14572-1-APDownload protocol
IHC protocol for TFAP2C antibody 14572-1-APDownload protocol
IP protocol for TFAP2C antibody 14572-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,ChIP

Mol Cancer

CircIL4R activates the PI3K/AKT signaling pathway via the miR-761/TRIM29/PHLPP1 axis and promotes proliferation and metastasis in colorectal cancer.

Authors - Tao Jiang
  • KD Validated
humanWB

Oncogene

Abrogation of glutathione peroxidase-1 drives EMT and chemoresistance in pancreatic cancer by activating ROS-mediated Akt/GSK3β/Snail signaling.

Authors - Qingcai Meng
humanchIP

Oncogene

TFAP2C regulates carbonic anhydrase XII in human breast cancer.

Authors - Christopher M Franke
humanWB

Front Oncol

TFAP2C-Mediated lncRNA PCAT1 Inhibits Ferroptosis in Docetaxel-Resistant Prostate Cancer Through c-Myc/miR-25-3p/SLC7A11 Signaling.

Authors - Xingkang Jiang
humanWB,IHC

Cancers (Basel)

TFAP2C Knockdown Sensitizes Bladder Cancer Cells to Cisplatin Treatment via Regulation of EGFR and NF-κB

Authors - Ji Xing
  • KD Validated
humanIHC

Nat Commun

Single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironment

Authors - Xiaojian Lu