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验证数据展示

  • FC experiment of MCF-7 using CL488-14572

    FC experiment of MCF-7 using CL488-14572

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TFAP2C (CL488-14572) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TFAP2C Polyclonal antibody

TFAP2C Polyclonal Antibody for FC (Intra)
Cat No. CL488-14572

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

hAP 2g, ERF1, AP2-gamma, AP2 GAMMA, ap 2gamma

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy TFAP2C Ready-To-Use IHC Kit. TFAP2C primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14572-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14572 targets TFAP2C in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen TFAP2C fusion protein Ag5648 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma)
Synonyms hAP 2g, ERF1, AP2-gamma, AP2 GAMMA, ap 2gamma
Calculated Molecular Weight 49 kDa
GenBank Accession NumberBC035664
Gene Symbol TFAP2C
Gene ID (NCBI) 7022
RRIDAB_2919096
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92754
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 TFAP2C antibody CL488-14572Download protocol
Standard Protocols
Click here to view our Standard Protocols