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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TFEB Polyclonal antibody

TFEB Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13372

Print datasheet

Synonyms

Transcription factor EB, TCFEB, Class E basic helix-loop-helix protein 35, bHLHe35, AlphaTFEB

验证数据展示

  • IF Staining of HepG2 using CL488-13372

    IF Staining of HepG2 using CL488-13372

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 TFEB antibody (CL488-13372) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TFEB Ready-To-Use IHC Kit. TFEB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13372-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13372 targets TFEB in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen TFEB fusion protein Ag4194 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transcription factor EB
Synonyms Transcription factor EB, TCFEB, Class E basic helix-loop-helix protein 35, bHLHe35, AlphaTFEB
Calculated Molecular Weight 476 aa, 53 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC032448
Gene Symbol TFEB
Gene ID (NCBI) 7942
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP19484
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calcualted molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa (PMID: 12837690). TFEB mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). Fisetin increases the levels of TFEB localized in nuclei (PMID: 27112200).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TFEB antibody CL488-13372Download protocol
Standard Protocols
Click here to view our Standard Protocols