验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy TFF1 Ready-To-Use IHC Kit. TFF1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83467-2-RR targets TFF1 in FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | FC (Intra), ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | TFF1 fusion protein Ag4541 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | trefoil factor 1 |
| 别名 | Polypeptide P1.A, PNR-2, pNR 2, HP1.A, Breast cancer estrogen-inducible protein |
| 计算分子量 | 84 aa, 9 kDa |
| GenBank蛋白编号 | BC032811 |
| 基因名称 | TFF1 |
| Gene ID (NCBI) | 7031 |
| RRID | AB_3671097 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P04155 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TFF1 (Trefoil factor 1, formerly known as pS2) is a small-secreted protein that belongs to the family of trefoil peptides, and characterized by the presence of one to six cysteine-rich P domains. TFF1 expression is frequently lost in gastric carcinoma, probably through mechanism of DNA methylation, and it is therefore considered as a tumor suppressor gene. The function of TFF1 was first controversially considered as an oncogene in breast cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for TFF1 antibody 83467-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |