验证数据展示
经过测试的应用
| Positive WB detected in | human stomach tissue, human urine sample |
| Positive IP detected in | mouse stomach tissue |
| Positive IHC detected in | human stomach tissue, human stomach cancer tissue, mouse stomach tissue, mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse stomach tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13681-1-AP targets TFF2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, hamster, gerbil |
| 免疫原 | TFF2 fusion protein Ag4537 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | trefoil factor 2 |
| 别名 | SML1, trefoil factor 2, Spasmolytic polypeptide, Spasmolysin, SP |
| 计算分子量 | 129 aa, 14 kDa |
| 观测分子量 | 18-20 kDa |
| GenBank蛋白编号 | BC032820 |
| 基因名称 | TFF2 |
| Gene ID (NCBI) | 7032 |
| RRID | AB_10598321 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q03403 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The trefoil factor family (TFF), comprises of three polypeptides, TFF1, TFF2 and TFF3 (7-12 kDa), secreted to mucosal surfaces by mucus producing cells, prominently in the gastrointestinal tract. TFF2, also known as spasmolytic polypeptide, is a low-molecular weight protein, expressed in mucous neck cells of the fundus and glands at the base of the antrum in normal human stomach. TFF2 could Inhibit gastrointestinal motility and gastric acid secretion. However, recent studies suggest that TFF2 could also play an important role in the immune system. We got a 18-20 kDa band in western botting maybe due to glycosylatation, and mature TFF2 is a 12 kDa protein (PMID: 10716671).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TFF2 antibody 13681-1-AP | Download protocol |
| IHC protocol for TFF2 antibody 13681-1-AP | Download protocol |
| IF protocol for TFF2 antibody 13681-1-AP | Download protocol |
| IP protocol for TFF2 antibody 13681-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Genet Systematic identification of cell-fate regulatory programs using a single-cell atlas of mouse development. | ||
Nat Cell Biol Lgr5-expressing chief cells drive epithelial regeneration and cancer in the oxyntic stomach. | ||
Nat Cell Biol A tumour-resident Lgr5+ stem-cell-like pool drives the establishment and progression of advanced gastric cancers. | ||
Gut Dissecting transcriptional heterogeneity in primary gastric adenocarcinoma by single cell RNA sequencing. | ||
Gastroenterology Induction of gastric cancer by successive oncogenic activation in the corpus. |
