验证数据展示
经过测试的应用
Positive IF-P detected in | mouse stomach tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-13681 targets TFF2 in IF-P applications and shows reactivity with human, mouse samples.
经测试应用 | IF-P Application Description |
经测试反应性 | human, mouse |
免疫原 | TFF2 fusion protein Ag4537 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | trefoil factor 2 |
别名 | SML1, SP, Spasmolysin, Spasmolytic polypeptide, TFF2, trefoil factor 2 |
计算分子量 | 129 aa, 14 kDa |
观测分子量 | 18-20 kDa |
GenBank蛋白编号 | BC032820 |
基因名称 | TFF2 |
Gene ID (NCBI) | 7032 |
RRID | AB_3083945 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q03403 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The trefoil factor family (TFF), comprises of three polypeptides, TFF1, TFF2 and TFF3 (7-12 kDa), secreted to mucosal surfaces by mucus producing cells, prominently in the gastrointestinal tract. TFF2, also known as spasmolytic polypeptide, is a low-molecular weight protein, expressed in mucous neck cells of the fundus and glands at the base of the antrum in normal human stomach. TFF2 could Inhibit gastrointestinal motility and gastric acid secretion. However, recent studies suggest that TFF2 could also play an important role in the immune system. We got a 18-20 kDa band in western botting maybe due to glycosylatation, and mature TFF2 is a 12 kDa protein (PMID: 10716671).
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 TFF2 antibody CL488-13681 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |