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验证数据展示

  • IF Staining of mouse stomach using CL488-13681

    IF Staining of mouse stomach using CL488-13681

    Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using CoraLite® Plus 488 TFF2 antibody (CL488-13681) at dilution of 1:200.

CoraLite® Plus 488-conjugated TFF2 Polyclonal antibody

TFF2 Polyclonal Antibody for IF-P
Cat No. CL488-13681

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P

Synonyms

SML1, SP, Spasmolysin, Spasmolytic polypeptide, TFF2, trefoil factor 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse stomach tissue
For other applications, we recommend the unconjugated version of this antibody, 13681-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13681 targets TFF2 in IF-P applications and shows reactivity with human, mouse samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse
Immunogen TFF2 fusion protein Ag4537 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name trefoil factor 2
Synonyms SML1, SP, Spasmolysin, Spasmolytic polypeptide, TFF2, trefoil factor 2
Calculated Molecular Weight 129 aa, 14 kDa
Observed Molecular Weight 18-20 kDa
GenBank Accession NumberBC032820
Gene Symbol TFF2
Gene ID (NCBI) 7032
RRIDAB_3083945
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ03403
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The trefoil factor family (TFF), comprises of three polypeptides, TFF1, TFF2 and TFF3 (7-12 kDa), secreted to mucosal surfaces by mucus producing cells, prominently in the gastrointestinal tract. TFF2, also known as spasmolytic polypeptide, is a low-molecular weight protein, expressed in mucous neck cells of the fundus and glands at the base of the antrum in normal human stomach. TFF2 could Inhibit gastrointestinal motility and gastric acid secretion. However, recent studies suggest that TFF2 could also play an important role in the immune system. We got a 18-20 kDa band in western botting maybe due to glycosylatation, and mature TFF2 is a 12 kDa protein (PMID: 10716671).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TFF2 antibody CL488-13681Download protocol
Standard Protocols
Click here to view our Standard Protocols